In typical complementary DNA microarray experiments, two fluorescently labeled RNAs are hybridized to an array of cDNA probes on a glass slide, and their relative gene expression intensities, ratios ...
The main steps involved and limitations of microarray analysis will be encountered. Two stages can be distinguished in microarray experiments: a pre-microarray experiment phase (tissue handling) and ...
Most public-founded large-scale sequencing projects that use cDNAs are primarily using cDNA libraries which are not enriched for full-length cDNAs. Consequently, only a fraction of the resulting ESTs ...
In this issue we take a close look at DNA microarrays, the current amore of biological and biomedical researchers. There's little reason to doubt that the infatuation will continue, at least for ...
Current microarray analysis uses 'chips' containing either 25-residue oligonucleotides synthesized by photolithography or cDNAs placed by robotic spotting. In the April Nature Biotechnology, Hughes et ...
The Nottingham Prognostic Index (NPI) applied to 467 patients (pts) with a long-term follow-up that were treated in a single institution No significant financial relationships to disclose. This is an ...
A significant limitation to the analytical accuracy and precision of dual-labeled spotted cDNA microarrays is the signal error due to dye bias. Transcript-dependent ...
We used cDNA microarrays to monitor changes in gene expression profiles induced by ionizing radiation in mouse total blood. The subsets of genes best characterizing each dose were identified by ...
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