In TIRF microscopy, fluorescent molecules are in a sample in an aqueous environment that is near a solid with a high refractive index, usually a glass coverslip. At what is called the critical angle, ...

Cool operator: the Saharan silver ant Cataglyphis bombycina. (Courtesy: P Landmann) A species of desert-dwelling ant is able to tolerate temperatures exceeding 50 °C because its body hairs act like ...

Refraction close refractionProcess by which a wave changes speed and sometimes direction upon entering a denser or less dense medium, eg a light ray changes direction when refracted by a lens. from a ...

If you want to visualize what happens at a biological interface or on the surface of a cell, it is vital to eliminate the background that comprises all the stuff that sits beyond that first layer.

When we first saw "interactive touch-screen display" in the tips jar, it made us think of playing MegaTouch 9000 at a seedy bar and we were about to toss it in the ol' recycling bin. Then we decided ...

The DeltaVision OMX V4 enables Total Internal Reflection Fluorescence Microscopy (TIRF). This technique uses an evanescent wave to selectively excite fluorophores that are close to the coverslip. This ...

The Wolfson Bioimaging Facility provides total internal reflection fluorescence microscopy (TIRF) with its multi-laser, dual-camera Olympus/Abbelight SAFe360 system which also offers single molecule ...