Nature1d
Regression of human metastatic renal cell carcinoma after vaccination with tumor cell–dendritic cell hybrids
Figure 1: Characterization of the hybrid cell vaccine. The hybrid cell vaccination was well tolerated by all patients. There were no serious adverse effects or any clinical signs of autoimmune ...
Nature22d
Flow cytometry smaller and better
Flow cytometry was thrust into the spotlight by the AIDS epidemic in the 1980s as it provides a quick and accurate way to count CD4 + and CD8 + T cells in HIV-infected patients. Until recently ...
thorax.bmj10d
Inflammation in preschool cystic fibrosis is of mixed phenotype, extends beyond the lung and is differentially modified by CFTR modulators
Background Early-life inflammation has long been recognised as a key pathophysiological process in the evolution of cystic ...
pharmaphorum13d
The critical bioanalytical strategies unlocking the potential of LNP-mRNA
The LNP-mRNA modality is a novel approach that uses lipid nanoparticles to deliver messenger RNA into cells. The mRNA acts as ...
ALZFORUM13d
From the Meninges, Regulatory T Cells Keep Brain Inflammation in Check
Stationed within the meninges, squads of immune cells lie in wait, ready to unleash an inflammatory assault within the brain if needed. Holding these trigger-happy troops back during peacetime falls ...
BMJ15d
Normal human enthesis harbours conventional CD4+ and CD8+ T cells with regulatory features and inducible IL-17A and TNF expression
Flow cytometry was used to further phenotype both blood and entheseal T cells. Entheseal-derived CD4+ and CD8+ T cells showed significantly higher CD69 expression, supporting a tissue resident memory ...
gut.bmj4d
O43 The phenotype and TCR repertoire of intestinal CD8+ T cells is altered in coeliac disease
Introduction Coeliac disease (CD) is a common immune-mediated condition driven by aberrant adaptive CD4+ T cell responses to gluten. The cytotoxic CD8+ and γδ+ T cell ... and 10x genomics protocols ...
Frontiers16d
Cytotoxic CD8+ T Cells Expressing CXCR5 Are Detectable in HIV-1 Elite Controllers After Prolonged In Vitro Peptide Stimulation
The association of EC with certain HLA-I alleles is one of the strongest indications for an involvement of anti-viral CD8 + T cells (5–7 ... Detailed cellular phenotyping of PBMCs using 18-color flow ...
Frontiers16d
Somatostatin-SSTR3-GSK3 modulates human T-cell responses by inhibiting OXPHOS
Antibodies used for flow cytometry staining in this study include: CD3-PerCp Cy5.5 (SK7, BD Bioscience), CD4-APC-Cy7 (RPA-T4, BD Bioscience), CD8-PE-Cy7 (RPA-T8, BD Bioscience), SSTR3-Alexa 647 ...
BMJ14d
NFIL3 mutations alter immune homeostasis and sensitise for arthritis pathology
Methods Here, we characterised a kindred of two monozygotic twin girls with juvenile idiopathic arthritis at the genetic and immunological level, using whole exome sequencing, single cell sequencing ...
technologynetworks21d
Mastering Fluorochrome Selection for Clearer Data
Hundreds of fluorochromes are available to users conducting flow cytometry assays, each with unique properties that influence panel resolution and data quality. Selecting the right combination can be ...
News Medical on MSN11d
How lymphoid tissues protect against viral infections
Researchers explore the cellular and molecular adaptations associated with human secondary lymphoid tissue in adults during ...