Physician's Weekly14d
Impact of PROC Variant C101F on Protein C Stability
The following is a summary of “Novel protein C variant p.C101F results in early intracellular degradation that drives type I ...
eLife10d
A dual ribosomal system in the zebrafish soma and germline
The hybrid-ribosome observation is convincing, but additional information on the choice of cycloheximide concentration would be helpful to rule out other interpretations. The experiment on translation ...
eLife10d
A dual ribosomal system in the zebrafish soma and germline
The deyolking buffer was supplemented with 1x protease inhibitors (EDTA-free, Roche) and 0.1 μg/mL cycloheximide. To avoid DNA contamination in the higher molecular weight factions, obtained larval ...
Frontiers11d
Altered Gene-Regulatory Function of KDM5C by a Novel Mutation Associated With Autism and Intellectual Disability
FIGURE 3. KDM5C R1115H is stable in cells. Lymphoblastoid cell lines from father (UM1-II-1, KDM5C WT) and proband (UM1-III-3, KDM5C R1115H) were treated with a cycloheximide (CHX) time course from 0 ...
Frontiers11d
Rapamycin inhibits hepatitis B virus covalently closed circular DNA transcription by enhancing the ubiquitination of HBx
Cycloheximide was purchased from Amresco (97064-722, WA, United States), and proteasome inhibitor MG132 (S2619) and Bortezomib (S1013) were acquired from Selleck Chemicals (Houston, United States).
jmg.bmj10d
Advancing in Schaaf-Yang syndrome pathophysiology: from bedside to subcellular analyses of truncated MAGEL2
Background Schaaf-Yang syndrome (SYS) is caused by truncating mutations in MAGEL2, mapping to the Prader-Willi region (15q11-q13), with an observed phenotype partially overlapping that of Prader-Willi ...